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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Droxinostat sensitizes human colon cancer cells to apoptotic cell death via induction of oxidative stress

Fig. 4

GT3 pretreatment blocked oxidative stress induced by droxinostat treatment in colon cancer cells. a – HT-29 cells were treated with GT3 followed by droxinostat treatment for 24 h. ROS production was measured using DCFDA and analyzed via flow cytometry. The levels of ROS are expressed as the means ± SD of three independent experiments. **p < 0.01 vs. vehicle, ##p < 0.01 vs. GT3. b – 500 HT-29 cells were seeded in 6-well plates and treated with or without GT3 and droxinostat. Ten days later, the colony numbers were counted and are expressed as the colony survival fraction. ***p < 0.001 vs. vehicle, ##p < 0.01 vs. GT3. C through E – HT-29 cells were treated with GT3 followed by droxinostat treatment for 24 h, and RNA was extracted to measure the expressions of catalase (c), SOD1 (d) and SOD2 (e) via RT-PCR. GADPH was used as a housekeeping control. *p < 0.001 vs. vehicle, **p < 0.001 vs. vehicle, ***p < 0.001 vs. vehicle, #p < 0.05 vs. GT3, ##p < 0.01 vs. GT3

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