Fig. 5
From: Oct4 regulates DNA methyltransferase 1 transcription by direct binding of the regulatory element
The Dnmt1 assay showed the decrease in Dnmt1 in CCE cells transfected with mouse Oct4 siRNA. a and b The mRNA (a) and protein (b) levels of Oct4 were downregulated by the three mouse Oct4 siRNA. c and d The final OD level (equal to the 450 nm OD minus the 655 nm OD) (c) and the amount of Dnmt1 (d) were reduced in mouse Oct4 siRNA R1-, R2-, and R3-transfected groups when compared with the negative control. Results are presented as the means ± SD. The final ODs from triplicate transfected samples were measured using microplate reader. * and **Statistically significant differences obtained with Student’s t-test for comparison with the negative control at p < 0.05 and 0.01