Fig. 3
From: MiR-320 regulates cardiomyocyte apoptosis induced by ischemia–reperfusion injury by targeting AKIP1
MiR-320 directly targets AKIP1. a Predicted binding sequences of miR-320 in AKIP1 3′ UTR. A fragment of wild-type (wt) or mutant (mut) 3′ UTR of AKIP1 was subcloned into the luciferase reporter vector. b H9c2 cells were co-transfected with miR-320 mimics and luciferase reporters that contained the predicted miRNA target site in the AKIP1 mRNA 3′ UTR or its corresponding mutant form. The cells co-transfected with miR control and luciferase reporters were used as the control group. The expression levels of AKIP1 mRNA and protein were measured by qRT-PCR (c) and Western blot (d), respectively. *P < 0.05