Fig. 6

Gap27 inhibits the mRNA expressions of IL-2 and IL-6 from Con A-stimulated PBMCs of spontaneously hypertensive rats (SHRs) and Wistar-Kyoto (WKY) rats. A set of PBMCs from SHRs and WKY rats were stimulated with Con A (5 μg/ml) for 24 h in culture medium. After pre-incubation with Gap27 (500 μM) for 48 h, a second set of PBMCs were incubated with Con A (5 μg/ml) for another 24 h. Both underwent quantitative real-time PCR analysis. The relative mRNA levels of IL-2 and IL-6 in PBMCs. GAPDH RNA was used as internal control. Values are the measn ± SEM from three independent experiments. **p < 0.01, compared with the control group in the same column (n = 5); ^##p < 0.01, compared with the Con A-stimulated group in the same column (n = 5)