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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: Hypoxic stabilization of mRNA is HIF-independent but requires mtROS

Fig. 5

mtROS production is sufficient for mRNA stabilization. HEK293T cells pre-treated with 10 μM of the Complex III Qi site inhibitor antimycin A and then exposed to 24 h of Normoxia or Hypoxia (1% O2) in the presence of 1 g/L or 4 g/L glucose before being treated with actinomycin D for 0, 1 or 2 h. mRNA levels of VEGF (a), MYC (b) and CYR61 (c) were determined via qPCR, normalized to GAPDH and 0 h time point and half-lives determined. Data represents average of N = 3 ± SD for each condition. *P ≤ 0.05 from 1 g/L normoxic control condition

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