Table 1 Primer sequences for PCR
From: The suppressive effect of co-inhibiting PD-1 and CTLA-4 expression on H22 hepatomas in mice
Gene name | Primer sequence |
|---|---|
β-actin | Sense: 5′-ATGGGTCAGAAGGACTCCTATG-3′ Antisense: 5′-ATCTCCTGCTCGAAGTCTAGAG-3′ |
PD-1 | Sense: 5′-GCCTGGCTCACAGTGTCAG-3′ Antisense: 5′-TCCAGGGCTCTCCTCGATT − 3′ |
CTLA-4 | Sense: GTCTTCTCTGAAGCCATACAG −3′ Antisense: 5′-GACCTCATCAGTGTTGTGTGA-3′ |
PD-L1 | Sense: 5′-GGAATTGTCTCAGAATGGTC-3′ Antisense: 5′-GTAGTTGCTTCTAGGAAGGAG-3′ |
IFN-γ | Sense: 5′-GCTTTGCAGCTCTTCCTCAT-3′ Antisense: 5′-GTCACCATCCTTTTGCCAGT-3′ |
IL-6 | Sense: 5′-CTTAATTACACATGTTCTCTGGGAAA-3′ Antisense: 5′-CAAGTGCATCATCGTTGTTCATAC-3′ |