Fig. 2

TSG inhibited PA-induced inflammation in H9c2 cardiomyocytes. PA-stimulated H9c2 cells with or without TSG (0.4 mM) for 48 h, TNF-α, IL-1β and IL-6 levels in the supernatant were measured by ELISA kit (a); RT-qPCR was performed to measure the mRNA expression of TNF-α, IL-1β and IL-6 (b); protein expression of NF-κB/p65 in the nucleus was measured by western blotting (c and d). ^* P < 0.05 compared with control group; ^# P < 0.05 compared with PA group. n = 3 in each group