Fig. 4

miR-129-3p loss-of-function reversed the protective effects of TSG in PA-induced inflammation and apoptosis. Expression of miR-129-3p was measured by RT-qPCR in H9c2 cells treated with PA (0.4 mM) or PA (0.4 mM) + TSG (0.4 mM) for 48 h (a). H9c2 cells transfected with miR-129-3p inhibitors and treated with PA (0.4 mM) and TSG (0.4 mM) for 48 h, cell apoptosis was detected by flow cytometry (b and c); RT-qPCR was performed to measure the mRNA expression of TNF-α, IL-1β and IL-6 (d). ^* P < 0.05 compared with control group; ^# P < 0.05 compared with PA or PA + TSG group. n = 3 in each group