Fig. 5

Smad3 is a direct target of miR-129-3p. The putative miR-129-3p binding sites in the 3′-UTR of Smad3 were predicted by on-line software (a). The luciferase activity assay was performed (b). After transfection with pre-miR-129-3p or anti-miR-129-3p, the mRNA expression of Smad3 (c) and protein expression of p-Smad3 (d) were assayed by RT-qPCR and western blotting, respectively. ^* P < 0.05 compared with pre-miR-Con group; ^# P < 0.05 compared with anti-miR-Con group. n = 3 in each group