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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: MiR-106a directly targets LIMK1 to inhibit proliferation and EMT of oral carcinoma cells

Fig. 2

The effects of LIMK1 silencing on the proliferation and EMT in OSCC cells. SCC4 cells were transfected with si-LIMK1 or si-NC for 48 h. a The mRNA and protein expressions of LIMK1 were determined via quantitative RT-PCR and western blot, respectively. b Cell proliferation was assessed with a BrdU-ELISA assay. c The mRNA expressions of PCNA, CDK2, CDK4, cyclin D1, cyclin E1, p21 and p27 were determined via quantitative RT-PCR. d The expressions of E-cadherin, N-cadherin, vimentin, SNAIL, SLUG and ZEB1 were determined via quantitative RT-PCR and western blot assays, respectively. All data are presented as means ± SEM, n = 6. #p < 0.05, ##p < 0.01 vs. si-NC

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