Fig. 2
From: MiR-106a directly targets LIMK1 to inhibit proliferation and EMT of oral carcinoma cells
The effects of LIMK1 silencing on the proliferation and EMT in OSCC cells. SCC4 cells were transfected with si-LIMK1 or si-NC for 48 h. a The mRNA and protein expressions of LIMK1 were determined via quantitative RT-PCR and western blot, respectively. b Cell proliferation was assessed with a BrdU-ELISA assay. c The mRNA expressions of PCNA, CDK2, CDK4, cyclin D1, cyclin E1, p21 and p27 were determined via quantitative RT-PCR. d The expressions of E-cadherin, N-cadherin, vimentin, SNAIL, SLUG and ZEB1 were determined via quantitative RT-PCR and western blot assays, respectively. All data are presented as means ± SEM, n = 6. #p < 0.05, ##p < 0.01 vs. si-NC