Fig. 6
From: MiR-106a directly targets LIMK1 to inhibit proliferation and EMT of oral carcinoma cells
Introduction of LIMK1 partially promoted cell proliferation and EMT in miR-106a-overexpressing OSCC cells. SCC4 cells were transfected with an miR-106a mimic with or without pcDNA-LIMK1 vector. a The mRNA and protein expressions of LIMK1 were determined via quantitative RT-PCR and western blot assays, respectively. b Cell proliferation was assessed using a BrdU-ELISA assay. c The mRNA expressions of PCNA, CDK2, CDK4, cyclin D1, cyclin E1, p21 and p27 were determined using quantitative RT-PCR. d The expressions of E-cadherin, N-cadherin, vimentin, SNAIL, SLUG and ZEB1 were determined via quantitative RT-PCR. All data are presented as means ± SEM, n = 6. #p < 0.05, ##p < 0.01, ###p < 0.001 vs. pcDNA3.1 + miR-106amimic