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Table 1 Sequence of primers for qRT-PCR

From: MiR-106a directly targets LIMK1 to inhibit proliferation and EMT of oral carcinoma cells

Gene Primer Sequence
LIMK1 F: 5’-CAAGGGACTGGTTATGGTGGC-3′
R: 5’-CCCCGTCACCGATAAAGGTC-3’
LIMK2 F: 5’-GGATTCCCTCACCAACTGGTA-3’
R: 5’-AGCCACCATAAAAGGCCCTG-3’
E-cadherin F: 5’-TACACTGCCCAGGAGCCAGA-3’
R: 5’-TGGCACCAGTGTCCGGATTA-3’
N-cadherin F: 5′- TCAGGCGTCTGTAGAGGCTT-3’
R: 5′- ATGCACATCCTTCGATAAGACTG-3’
Vimentin F: 5’-GACGCCATCAACACCGAGTT-3’
R: 5’-CTTTGTCGTTGGTTAGCTGGT-3’
Snail F: 5’-TCGGAAGCCTAACTACAGCGA-3’
R: 5’-AGATGAGCATTGGCAGCGAG-3’
Slug F: 5’-CGAACTGGACACACATACAGTG-3’
R: 5’-CTGAGGATCTCTGGTTGTGGT-3’
ZEB1 F: 5’-GATGATGAATGCGAGTCAGATGC-3’
R: 5’-ACAGCAGTGTCTTGTTGTTGT-3’
PCNA F: 5’-CCTGCTGGGATATTAGCTCCA-3’
R: 5’-CAGCGGTAGGTGTCGAAGC-3’
CDK2 F: 5’-TGTTTAACGACTTTGGACCGC-3’
R: 5’-CCATCTCCTCTATGACTGACAGC-3’
CDK4 F: 5’-GGGGACCTAGAGCAACTTACT-3’
R: 5’-CAGCGCAGTCCTTCCAAAT-3’
cyclin D1 F: 5’-GCTGCGAAGTGGAAACCATC-3’
R: 5’-CCTCCTTCTGCACACATTTGAA-3’
cyclin E1 F: 5’-AAGGAGCGGGACACCATGA-3’
R: 5’-ACGGTCACGTTTGCCTTCC-3’
p21 F: 5’-TGTCCGTCAGAACCCATGC-3’
R: 5’-AAAGTCGAAGTTCCATCGCTC-3’
p27 F: 5’-AACGTGCGAGTGTCTAACGG-3’
R: 5’-CCCTCTAGGGGTTTGTGATTCT-3’
ZEB2 F: 5’-CAAGAGGCGCAAACAAGCC-3’
R: 5’-GGTTGGCAATACCGTCATCC-3’
U6 F: 5’-CTCGCTTCGGCAGCACA-3’
R: 5’-AACGCTTCACGAATTTGCGT-3’
GAPDH F: 5’-GAGTCAACGGATTTGGTCGTATTG-3’
R: 5’-CCTGGAAGATGGTGATGGGATT-3’