Fig. 5

Up-regulation of miR-613 suppressed invasion of RASFs. RASFs cells were transfected with miR-613 or miR-NC for 48 h, and then seeded in the top chamber. (a) After 6 h, the RASFs that invaded through the membrane were stained and quantified. (b) Levels of MMP-2 and -9 were detected in the culture supernatants of cultured RASFs by ELISA assay. (c) mRNA levels of MMP-2 and -9 were examined by qRT-PCR. (d) Protein expression of MMP-2 and -9 was determined by Western blotting. All data are presented as mean ± SEM, n = 6. ^#P < 0.05, ^##P < 0.01 vs. miR-NC