Fig. 7From: Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cellsCell viability assay and tube formation assay with human umbilical vein endothelial cells (HUVECs). HUVECs were cultured in conditioned medium of cultivated oral mucosal epithelial cells (COMECs) co-cultured with LNCs (CM-LNCs) and 3 T3 cells (CM-3T3s). Cell viability (a) and tube formation (b, c) of HUVECs were assessed. HUVECs cultured in Endothelial Cell Medium (ECM group) were used as control. c Micrographs of the tube-like structures in ECM group, CM-LNCs and CM-3T3s were captured. The results were expressed as mean ± standard deviation. LNCs: limbal niche cells, scale bars: 200 μm, *: p < 0.05Back to article page