Table 2 Primer sequences used for RT-qPCR
From: Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells
Name | Primer | Sequence | Size |
|---|---|---|---|
β-actin | Forward | CACGATGGAGGGGCCGGACTCATC | 240 bp |
Reverse | TAAAGACCTCTATGCCAACACAGT | ||
GAPDH | Forward | ACAGCAACAGGGTGGTGGAC | 252 bp |
Reverse | TTTGAGGGTGCAGCGAACTT | ||
Rat bFGF | Forward | AGCATCACTTCGCTTCCCGC | 226 bp |
Reverse | GGTTCGCACACACTCCCTTG | ||
Rat PEDF | Forward | GAAGGCGACGTTACCAACTC | 242 bp |
Reverse | TCCGTGTCCCTCAGAACAAA | ||
Rat sFlt-1 | Forward | CCCTCAGCCTACCATCAAGT | 234 bp |
Reverse | GCCTTGCAGCTGTAGATTCC | ||
Rat VEGF | Forward | CGTCTACCAGCGCAGCTATTG | 145 bp |
Reverse | CTCCAGGGCTTCATCATTGC | ||
Rat TSP-1 | Forward | GTGTCAGTGGAGGAAGCTCT | 193 bp |
Reverse | TGACATCTCCCTTTGCGACT | ||
Rat TIMP-3 | Forward | GCCCTTTGGCACTCTGGTCT | 151 bp |
Reverse | TGTCAGCAGGTACTGGTATTTGT | ||
Rat Endostatin | Forward | CCGTGCCCATCGTCAACCT | 217 bp |
Reverse | GTCCGCCACGTCTCACAGTAG |