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Table 2 Primer sequences used for RT-qPCR

From: Limbal niche cells can reduce the angiogenic potential of cultivated oral mucosal epithelial cells

Name Primer Sequence Size
β-actin Forward CACGATGGAGGGGCCGGACTCATC 240 bp
Reverse TAAAGACCTCTATGCCAACACAGT
GAPDH Forward ACAGCAACAGGGTGGTGGAC 252 bp
Reverse TTTGAGGGTGCAGCGAACTT
Rat bFGF Forward AGCATCACTTCGCTTCCCGC 226 bp
Reverse GGTTCGCACACACTCCCTTG
Rat PEDF Forward GAAGGCGACGTTACCAACTC 242 bp
Reverse TCCGTGTCCCTCAGAACAAA
Rat sFlt-1 Forward CCCTCAGCCTACCATCAAGT 234 bp
Reverse GCCTTGCAGCTGTAGATTCC
Rat VEGF Forward CGTCTACCAGCGCAGCTATTG 145 bp
Reverse CTCCAGGGCTTCATCATTGC
Rat TSP-1 Forward GTGTCAGTGGAGGAAGCTCT 193 bp
Reverse TGACATCTCCCTTTGCGACT
Rat TIMP-3 Forward GCCCTTTGGCACTCTGGTCT 151 bp
Reverse TGTCAGCAGGTACTGGTATTTGT
Rat Endostatin Forward CCGTGCCCATCGTCAACCT 217 bp
Reverse GTCCGCCACGTCTCACAGTAG
  1. GAPDH: glyceraldehyde 3-phosphate dehydrogenase, bFGF: basic fibroblast growth factor, PEDF: pigment epithelium-derived factor, sFlt-1: soluble fms-like tyrosine kinase-1, VEGF: vascular endothelial growth factor, TSP-1: thrombospondin-1, TIMP-3: tissue inhibitor of metalloproteinase-3