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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: MicroRNA-325-3p inhibits cell proliferation and induces apoptosis in hepatitis B virus-related hepatocellular carcinoma by down-regulation of aquaporin 5

Fig. 2

AQP5 was a direct target of miR-325-3p. a Levels of miR-325-3p in normal liver tissues (n = 20) and HBV-HCC tissues (n = 20) were detected by qRT-PCR. b Levels of miR-325-3p in HepG2, HepG2.2.15, Huh7 and Huh7–1.3 cells. c Pearson’s correlation analysis of the relative expression levels of miR-325-3p and the relative AQP5 mRNA levels in HBV-HCC tissues. d Schematic representation of AQP5 3′-UTRs showing putative miRNA target site. e mRNA expression of AQP5 was determined by qRT-PCR in Huh7–1.3 and HepG2.2.15 cells transfected with miR-325-3p mimic or miR-NC. f Protein expression of AQP5 was determined by Western blot in Huh7–1.3 and HepG2.2.15 cells transfected with miR-325-3p mimic or miR-NC. g Analysis of the relative luciferase activities of AQP5-WT and AQP5-MUT in Huh7–1.3 and HepG2.2.15 cells. All data are presented as mean ± SEM, n = 6. ***P < 0.001 vs. normal tissues; ##p < 0.01, ###p < 0.001 vs. HepG2, Huh7 or miR-NC

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