Fig. 5

Effects of AQP5 overexpression on cell proliferation and apoptosis in Huh7–1.3 and HepG2.2.15 cells transfected with miR-325-3p mimic. Huh7–1.3 and HepG2.2.15 cells were transfected with miR-325-3p mimic and pcDNA-AQP5 or pcDNA3.1. Expression of AQP5 was detected by Western blot (a) and qRT-PCR (b). Cell proliferation was assessed by CCK-8 assay (c) and BrdU-ELISA assay (d). e Cell apoptosis was measured by nucleosomal degradation using Roche’s cell death ELISA detection kit. f The activities of caspase-3 were determined by caspase-3 activity detection assay. All data are presented as mean ± SEM, n = 6. ^# p < 0.05, ^## p < 0.01, ^### p < 0.001 vs. miR-325-3p mimic + pcDNA3.1