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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: MiR-214 sensitizes human colon cancer cells to 5-FU by targeting Hsp27

Fig. 1

miR-214 sensitizes colon cancer cells to 5-FU. a – IC50 values for 5-FU from HT-29/5-FU, LoVo/5-FU and their parental cells were determined using the CCK-8 assay. b – The differential miRNA expressional profiles from HT-29, HT-29/5-FU, LoVo and LoVo/5-FU cells determined using microarray assays. c –Quantification of miRNA expression inHT-29, HT-29/5-FU, LoVo and LoVo/5-FU cells was performed via qPCR to validate the results of the microarrays. d and e – HT-29 and LoVo cells were both transfected with miR-214 ASO and control. After incubation with 5-FU at concentrations of 0.0016, 0.008, 0.04, 0.2 and 1.5 μg/ml for 48 h, inhibitions were detected using CCK-8 assays, and the IC50 values were determined. F and G – HT-29/5-FU and LoVo/5-FU cells were both transfected with miR-214 mimics and controls. Inhibitions (f) and IC50 values (g) were determined as in d and e. Data represent the means ± SEM of three independent experiments. *p < 0.05

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