Fig. 2

miR-214 sensitizes colon cancer cells to 5-FU by activating caspase-3. a and b –HT-29 and LoVo cells were transfected with miR-214 ASO and ASO control and harvested 48 h after transfection. Colony formation assays were done to detect cell growth after 14 days of culture. c and d –Relative OD values were determined using CCK-8 assays after 1, 2, 3, 4, 5 and 6 days in HT-29 and LoVo cells. e and f – Cells were incubated with 0.05 μg/ml of 5-FU for 48 h. TUNEL assays were performed to detect cell apoptosis. The blue stain is the cell nucleus; green is TUNEL staining representing apoptotic cells (scale bar = 100 μm). g – Western blots were done to detect caspase-3 protein levels. Data represent the means ± SEM from three independentexperiments. *p < 0.05