Fig. 3

miR-214 targets Hsp27 by binding to the 3’UTR. a – The complementary site of miR-214 in the 3’UTR of Hsp27 as predicated using bioinformatic analysis. b – Hsp27 3’UTR wtand Hsp27 3’UTR mutant luciferase constructs were simultaneously transfected into cells with miR-214 ASO, miR-214 mimics or corresponding controls. Luciferase activities were measured at 48 h post-transfection. c – Western blots were done to detect the protein levels of Hsp27, p-Hsp27, p-P38 MAPK and P38 MAPK in the cells. GAPDH was used as the internal control protein. d – Hsp27 protein levels in miR-214 ASO or miR-214 mimics transfected into HT-29 and LoVo cells were measured viawestern blot. Data represent the means ± SEM from three independentexperiments. *p < 0.05