Fig. 4

Hsp27 affects the sensitivity of colon cancer cells to 5-FU. a, b and c – HT-29 and LoVo cells were transfected with pcDNA3-Hsp27 or pcDNA3 controls. Hsp27 protein expression was detected at 48 h post-transfection via western blot (a). After cells were incubated with 5-FU at concentrations of 0.0016, 0.008, 0.04, 0.2 and 1.5 μg/ml for 48 h,IC₅₀ values were determined using CCK-8 assays (b). After the cells were incubated with 0.05 μg/ml 5-FU for 48 h, cell apoptosiswas determined using TUNEL assays (c, scale bar = 100 μm). d, e and f – HT-29 and LoVo cells were transfected with Hsp27 siRNA or siRNA controls. Hsp27 protein expression (d), IC₅₀ (e) and cell apoptosis (f, scale bar = 100 μm) were assessed. Data represent the means ± SEM from three independentexperiments. *p < 0.05