Fig. 2

Expression levels of Hedgehog proteins and Hedgehog signaling molecules. (a and c) qRT-PCR was used to demonstrate mRNA expression levels of IHH and SHH following viral transfection; values are means ± SD (n = 3). The results were normalized to B2M mRNA expression. (b and d) ELISA was used to demonstrate IHH and SHH expression levels following viral transfection. Values are means ± SD (n = 5). (e) The hedgehog signaling molecules Ptc, Smo and Gli1 were detected by western blotting after viral transfection for 72 h. (f) The results were normalized to GAPDH protein expression. Values are means ± SD (n = 3). Significant differences between the control group (non-transfection cells) are indicated by *p < 0.05 or ** p < 0.01; differences between IHH and SHH transfection groups are indicated by ^# p < 0.05 or ^## p < 0.01