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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system

Fig. 4

Expression levels of related genes during differentiation induction in the RCCS environment. (a) qPCR analysis of Sox9, ACAN and collagen II on days 7, 14 and 21 during induction. (b) qRT-PCR analysis of collagen X, Runx2, and annexin V on days 7, 14 and 21 during induction. The results were normalized to B2M mRNA expression. Values are means ± SD (n = 3). (c) Expression of ACAN, collagen II, collagen X and Runx2 was detected by western blotting on day 10 during differentiation induction. (d) Expression of ACAN, collagen II, collagen X and Runx2 was detected by western blotting on day 21 during differentiation induction. Significant differences from the control group (non-transfection cells) are indicated by *p < 0.05 or ** p < 0.01; differences between IHH and SHH transfection groups are indicated by # p < 0.05 or ## p < 0.01

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