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Table 1 Primer sequences for quantitative real-time polymerase chain reaction

From: Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system

Gene

Forward primer

Reverse primer

IHH

CCAACTACAATCCCGACATCATCT

CTCGTCCCAGCCTTCAGTCA

SHH

CTGACCGTGACCGTAGCAAGT

TGGATGTGGGCTTTGGACTCA

Sox9

CTGACCGTGACCGTAGCAAGT

TGGATGTGGGCTTTGGACTCA

ANCN

ATGGCTTCCACCAGTGCG

CGGATGCCGTAGGTTCTCA

collagen II

GCTCCCAGAACATCACCTACCA

ATTCCTGCTCAGGCCCTCC

collagen X

CCCTTCTGCTGCTAGTGTC

GTCTTGGTGTTGGGTTGT

Runx2

CCTTCCACTCTCAGTAAGAAGA

TAAGTAAAGGTGGCTGGATAGT

annexin V

GCAGAACTAACAGCCATAA

AGAACCACCAACATCCTC

Beta-2-microglobulin

AACGTGGAACAGTCAGACC

AGTAATCTCGATCCCATTTC

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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