Fig. 2

MiR-125a-5p regulates osteoclastogenesis from RAW 264.7 cells. a RAW 264.7 cells were induced with M-CSF and RANKL for 3 days. The relative miR-125a-5p levels were determined by qRT-PCR after treatment with miR-125a-5p mimics or anti-miR-125a-5p; b Relative TRAP, MMP-2, MMP-9, and cathepsin K mRNA levels were determined by qRT-PCR and normalized to GAPDH; c The expression levels of TRAP, MMP-2, MMP-9, and cathepsin K were determined by western blotting assay; d Representative photographs of osteoclastogenesis and the number of TRAP-positive multinucleated cells are shown. The data represent the mean ± SD of three experiments in triplicate. ^** P < 0.01 compared with control