Fig. 3

MiR-125a-5p increases osteoclast precursor proliferation, motility and invasion. a Cells were cultured for 3 days in the presence of M-CSF and RANKL. The proliferation of RAW 264.7 cells was determined by MTT assay; b The migration of osteoclast precursors was measured using wound healing assay. Scale bars indicate 100 μm; c The invasion of RAW 264.7 cell was determined using Transwell invasion assay. Data are mean ± SD, representative of at least three independent experiments performed in triplicate; d The expression of MMP-2 and MMP-9 of RAW 264.7 cells treated with miR-125a-5p or anti-miR-125a-5p was analyzed by immunofluorescence assay. ** P < 0.01 versus control