Fig. 4

MiR-125a-5p directly regulates the expression of TNFRSF1B in RAW 264.7 cells. a The complementary sequences of miR-125a-5p were discovered in 3′-UTR of TNFRSF1B mRNA using TargetScan, miRTarBase and miRDB. Venn graph represents the number of candidate common target genes determined by three bioinformatics analyses. b RAW 264.7 cells were transfected with miR-NC or miR-125a-5p and the levels of potential target genes were measured by qRT-PCR assay. c The complementary sequences of miR-125a-5p were discovered in 3′-UTR of TNFRSF1B mRNA using TargetScan. The mutagenesis was performed in the complementary sites for the seed region of miR-125a-5p (wt, wild type; mut, mutant type). d RAW 264.7 cells were transfected with miR-125a-5p or miR-NC. Immunofluorescence assay indicated that up-regulation of miR-125a-5p reduced the expression of TNFRSF1B. e MiR-125a-5p inversely modulated the luciferase activity of plasmids containing wt 3′-UTR of TNFRSF1B. ^** P < 0.01 compared to miR-NC