Fig. 7

MiR-125a-5p promotes osteoclast differentiation through targeting TNFRSF1B. a RAW 264.7 cells were treated with miR-125a-5p mimics or miR-125a-5p mimics with pcDNA3.1(+) containing TNFRSF1B. The expression of TNFRSF1B was assessed using qRT-PCR assay; b RAW 264.7 cells were treated with miR-125a-5p mimics or miR-125a-5p mimics with pcDNA3.1(+) containing TNFRSF1B. The expression of TNFRSF1B was assessed using immunofluorescence assay; c RAW 264.7 cells were treated with miR-125a-5p mimics or miR-125a-5p mimics with pcDNA3.1(+) containing TNFRSF1B in the presence of M-CSF + RANKL. TRAP-positive (pink to purple) multinucleated cells with more than three nuclei were counted as mature osteoclasts; d RAW 264.7 cells were treated with miR-125a-5p mimics or miR-125a-5p mimics with pcDNA3.1(+) containing TNFRSF1B. The mRNA levels of TRAP, MMP-2, MMP-9, and cathepsin K were determined using qRT-PCR assay; e The protein expression of TRAP, MMP-2, MMP-9, and cathepsin K in RAW 264.7 cells was determined by western blot assay. ^** P < 0.01 compared to control, ^## P < 0.01 compared to miR-125a-5p