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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells

Fig. 1

Immunolocalization of MTNR1A and MTNR1B in mouse testes and mLTC-1 cells. a. Control staining was performed without primary antibody but PBS. b. Immunohistochemistry staining showed that MTNR1A was specially labeled in Leydig cell and Sertoli cells in mouse testes. c. Immunohistochemistry staining showed that MTNR1B was specially labeled in Leydig cells and Sertoli cells in mouse testes. d-f. Immunofluorescence staining using anti-MTNR1A and MTNR1B antibody and Alexa-Fluor 488 labeled secondary antibody showed that both MTNR1A and MTNR1B expressed specifically on cell membrane in mLTC-1 cells. Scale bar: 50 μm

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