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Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: Melatonin receptor depletion suppressed hCG-induced testosterone expression in mouse Leydig cells

Fig. 2

siRNA-mediated knockdown of MTNR1A and MTNR1B in mLTC-1 cells. a and b. qPCR assay of the MTNR1A and MTNR1B mRNA levels in mLTC-1 after transfection of siRNAs against MTNR1A and MTNR1B, respectively. c and d. Western blotting results of MTNR1A and MTNR1B protein levels in mLTC-1 after transfection of siRNAs against MTNR1A and MTNR1B, respectively. e and f. The intensity of WB bands in C and D was evaluated with ImageJ. Data are presented as mean ± SEM of triplicate experiments. * P < 0.05, ** P < 0.01, *** P < 0.001 (Student’s t-test)

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