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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: Upregulation of miR-29b-3p protects cardiomyocytes from hypoxia-induced apoptosis by targeting TRAF5

Fig. 3

MiR-29b-3p targets the 3’UTR of TRAF5. a – The predicted miR-29b-3p-binding sites located in the 3’UTR of TRAF5. b – The effect of miR-29b-3p on luciferase activity was detected using the dual-luciferase reporter assay. The pmirGLO reporter vector containing the WT or MUT TRAF5 was co-transfected with miR-29b-3p or miR-NC into AC16 cells for 48 h. c and d – The mRNA and protein expressions of TRAF5 in AC16 cells transfected with miR-29b-3p or miR-NC and then subjected to hypoxia for 24 h were determined using quantitative real time PCR and western blot analysis. Each experiment was repeated three times. ***p < 0.001

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