Fig. 1

Screening for miRNAs that target the 3′-UTR of HIF-1α. a – Schematic depiction of the TargetScan predictions for the binding sites for miRNAs in the 3′-UTR of HIF-1α. The locations of miRNA-binding sites in the 3′-UTR of the HIF-1α gene are represented by numbers in parentheses. b – Luciferase reporter assay. A549 cells were co-transfected in duplicate with 3′-UTR-luciferase reporter plasmid and miRNA mimics. The luciferase activity was measured 48 h post-transfection. Luciferase activity in NC-transfected cells was set at 100%