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Fig. 3 | Cellular & Molecular Biology Letters

Fig. 3

From: MiR-107 function as a tumor suppressor gene in colorectal cancer by targeting transferrin receptor 1

Fig. 3

MiR-107 directly targeting at TFR1. a The sequence of human miR-107 and the predicted binding sites with miR-107 within the TFR1 3′-UTR are shown. b Photos of western blots showing that miR-107 inhibited the expression of miR-107 in SW620 cells. SW620 cell were treated with NC mimic or miR-107 mimic for 24 h. Then, the cells were collected and subjected to western blotting assay. c MiR-107 suppressed the transcription of TFR1 mRNA. SW620 cells were co-transfected with luciferase plasmids containing wild-type TFR1 3′-UTR or mutational TFR1 3′-UTR. The cells were also treated with NC mimic or miR-107 mimic. The cells were collected and lysed to measure the relative luciferase activity. Quantitative data are presented as the means ± SEM. *** p < 0.001 compared with the NC mimic group. d The TFR1 mRNA level in human colorectal cancer and the corresponding mucosa tissues. Quantitative data are presented as the means ± SEM, n = 50. *** p < 0.001 compared with normal tissues. e The TFR1 expressions in human colorectal cancer lines and normal human colon epithelial cell HCoEpiC were analyzed with RT-PCR. The TFR1 expression in HCoEpiC was set as 100%. Quantitative data are presented as the means ± SEM. *** p < 0.001 compared with BEA-2B cells. f The miR-107 expression in human colorectal cancer tissues is negatively correlated with that of TFR1. R2 stands for goodness of fit and p stands for significance of the slope

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