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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: Curcumin suppresses epithelial-to-mesenchymal transition of peritoneal mesothelial cells (HMrSV5) through regulation of transforming growth factor-activated kinase 1 (TAK1)

Fig. 1

Effect of curcumin on HMrSV5 cell viability and migration. Cells were treated with various concentrations of curcumin (10, 20, 40 and 80 μM) for 12, 24, 48 and 72 h (a) or various concentrations of PDS (1.5% Dianeal, 2.5% Dianeal, 4.25% Dianeal) for 24 h (b) or PDS (4.25% Dianeal) for various times (0, 12, 24 and 48 h) (c). Cells were divided into control group (cells treated with DMEM/F12 medium containing 0.5%FBS), 4.25% Dianeal group, and 4.25% Dianeal + curcumin group (cells treated with 4.25% Dianeal and curcumin of 20, 40, and 80 μM) (d). Cell viability was measured by CKK-8 assay. *P < 0.05. Effect of curcumin on HMrSV5 cell migration was determined by Transwell assay, and the migrating cells were detected by crystal violet staining (e: control group; f: 4.25% Dianeal group; g: 4.25% Dianeal + curcumin 20 μM; h: 4.25%D + curcumin 40 μM; i: 4.25% Dianeal + curcumin 80 μM; j: curcumin 80 μM). Six random fields for each slice were counted, n = 3. *P < 0.05 vs. control group; #P < 0.05 vs. 4.25% Dianeal group k: The quantification of the migrating cells is expressed graphically

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