Fig. 2

Effect of curcumin on PD-induced mRNA expressions of EMT-related genes in HMrSV5 cells. Cells were divided into control group (cells treated with DMEM/F12 medium containing 0.5%FBS), three PD groups (cells treated with 1.5% Dianeal, 2.5% Dianeal and 4.25% Dianeal), and three 4.25% Dianeal + curcumin group (cells treated with 4.25% Dianeal plus curcumin of 20, 40 and 80 μM). After incubation for 48 h, cells were lysed using TRIzol reagent for quantitative real-time PCR assay, and culture supernatant was collected for ELISA assay. GAPDH served as a loading control. Relative mRNA expressions of E-cadherin (a), α⁃SMA (b), collagen I (c), fibronectin (d) and TGF⁃β1 (e) are shown. TGF-β1 protein content in culture supernatant was determined (f). *P < 0.05 vs. control group; #P < 0.05 vs. 4.25% Dianeal group