Fig. 3

Effect of curcumin on PD-induced EMT marker protein expressions in HMrSV5 cells. Cells were divided into control group (cells treated with DMEM/F12 medium containing 0.5%FBS), three PD groups (cells treated with 1.5% Dianeal, 2.5% Dianeal and 4.25% Dianeal) and three 4.25% Dianeal + curcumin group (cells treated with 4.25% Dianeal plus curcumin of 20, 40 and 80 μM). After incubation for 48 h, cells were lysed using RIPA buffer for western blot assay. Representative immunoblots of α-SMA, E-cadherin and β-actin in HMrSV5 cells under various treatments are shown (a, b). β-actin served as a loading control. The density of protein bands were converted into grayscale values and are expressed as mean ± SEM. Relative protein expressions of α-SMA (c, d) and E-cadherin (e, f) are normalized to those of β-actin in HMrSV5 cells under various treatments. *P < 0.05 vs. control group; #P < 0.05 vs. 4.25% Dianeal group