Fig. 2

Transcriptional induction of TFEB and CLEAR network genes in cells subjected to treatment with ammonium chloride and upon overexpression of TFEB. a Expression levels of TFEB transcripts in ARPE-19 cells treated with ammonium chloride. b The expression of autophagy and lysosomal genes: ATP6V0A1, BECN1, LAMP1 and p62was analyzed by qRT-PCR in cells treated with ammonium chloride. c ARPE-19 cells were transfected with a constitutively active mutant of TFEB (S142A; S211A) and the cellular levels of TFEB transcripts was analyzed by qRT-PCR. d qRT-PCR analysis of the expression of lysosomal (LAMP-1, CTSD, MCOLN1 and ATP6V0A1) and autophagy genes (BECN1, MAP1LC3B and p62) upon overexpression of constitutively active TFEB in ARPE-19 cells. Values represent mean ± s.d. of three independent experiments. Student’s t-test (two tailed) was used for analysis *P-value <0.05; **P-value <0.01