Fig. 3
From: MicroRNA-340-5p suppresses non-small cell lung cancer cell growth and metastasis by targeting ZNF503
MiR-340-5p directly targets ZNF503 in NCI-H1650 cells. a The presumptive binding sites of miR-340-5p within the WT-3′-UTR-ZNF503 and the Mut-3′-UTR-ZNF503 are shown. b A dual luciferase reporter assay was used to evaluate the binding specificity between miR-340-5p and WT-3′-UTR-ZNF503. c The mRNA level of ZNF503 in NCI-H1650 cells transfected with miR-340-5p mimics was determined using a qPCR assay. d The expression of ZNF503 protein was determined via western blotting after NCI-H1650 cells were transfected with miR-340-5p mimics. e The relative protein expression is shown. Data are expressed as the means ± SD (n = 3). *p < 0.05 and **p < 0.001 versus the scrambled group