Fig. 5

Addition of TCTN1 reversed miR-216a-5p-mediated effects on cell proliferation and apoptosis. EC9706 cells were co-transfected with miR-216a-5p mimic/miR-NC and with TCTN1 overexpression plasmid/empty vector. a CCK-8 assay was used to analyze cell proliferation. b Flow cytometry with double Annexin V/PI staining was used to detect cell apoptosis. **p < 0.01, ***p < 0.001 vs. siNC+vector; #p < 0.05, ##p < 0.01 vs. miR-216a-5p + vector