Fig. 3
From: The effect of AP-2δ on transcription of the Prestin gene in HEI-OC1 cells upon oxidative stress
AP-2δ bound to Prestin in HEI-OC1 cells, as validated by ChIP assay. a The product of two primers flanking the transcriptional start site (TSS) of Prestin (S-1441 and S-784) was subjected to agarose gel electrophoresis after PCR. An input group, IP group, and IgG group were set up for each site. b Enrichment analysis of AP-2δ ChIP on Prestin regulatory regions. GAPDH was used as an endogenous control. Enrichment of AP-2δ-bound Prestin amount was normalized to corresponding amount in input group. The data were presented as the mean ± SD, n = 3 each group. * and # represent P < 0.05 compared with the IgG group of S-1441 and the IP group of S-784 respectively