Fig. 4
From: The effect of AP-2δ on transcription of the Prestin gene in HEI-OC1 cells upon oxidative stress
The siTfap2d-b fragment was characterized by the best specificity in knocking down AP-2δ. a HEI-OC1 cells were transfected with three siTfap2d fragments for 24 h and subjected to qRT-PCR to detect the expression level of AP-2δ mRNA. b Representative western blot of AP-2δ from cells treated with siTfap2d. GAPDH was used as an endogenous control. These images came from the same gel. c Expression level of AP-2δ protein in HEI-OC1 cells treated with siTfap2d. The data were normalized to GAPDH expression, and they were presented as the means±SD; n = 3 each group. * and # represent P < 0.05 compared with the group treated with the siScrambled fragment and the group treated with the siTfap2d-b fragment respectively