Fig. 5
From: The effect of AP-2δ on transcription of the Prestin gene in HEI-OC1 cells upon oxidative stress
AP-2δ negatively regulated the transcription of Prestin. a HEI-OC1 cells were transfected with the siTfap2d-b fragment for 24 h and subjected to qRT-PCR to detect the expression level of AP-2δ and Prestin mRNA. b Representative western blot of AP-2δ and Prestin from cells treated with siTfap2d-b. GAPDH was used as an endogenous control. These images came from the same gel. c Expression level of AP-2δ and Prestin protein in HEI-OC1 cells treated with siTfap2d-b. The data were normalized to GAPDH expression, and they were presented as the means±SD; n = 3 each group. * represents P < 0.05 compared with the group treated with the siScrambled fragment