Table 1 gRNA sequences
From: The effects of CRISPR-Cas9 knockout of the TGF-β1 gene on antler cartilage cells in vitro
Name | Sequence (5′ to 3′) |
|---|---|
gRNA1-F | caccgGGTGAAGCGGAAGCGCATCG |
gRNA1-R | aaacCGATGCGCTTCCGCTTCACCc |
gRNA2-F | caccgTTTACAACAGTACCCGCGAC |
gRNA2-R | aaacGTCGCGGGTACTGTTGTAAAc |
gRNA3-F | caccgTTGGCGTAGTAGTCCGCCTC |
gRNA3-R | aaacGAGGCGGACTACTACGCCAAc |