Fig. 2

The levels of ALP and calcification after miR-135-5p overexpression or knockdown during osteoblast differentiation of MC3T3-E1 cells. a – The expressions of miR-135-5p after MC3T3-E1 cells were treated with 300 ng/ml BMP2 for osteogenic induction (determined using quantitative RT-PCR). *p < 0.05, **p < 0.01, ***p < 0.001 vs. day 0. b – The expression of miR-135-5p after MC3T3-E1 cells were transfected with miR-135-5p mimic or miR-135-5p inhibitor (determined using quantitative RT-PCR). ***p < 0.001 vs. mimic control; ^##p < 0.01 vs. inhibitor control. c – The level of ALP was measured using an ALP assay kit. d – The area stained with alizarin red staining was quantified. e – The level of calcification was measured using alizarin red staining. ***p < 0.001 vs. control; ^##p < 0.01, ^###p < 0.001 vs. mimic control; ^△△p < 0.01, ^△△△p < 0.001 vs. inhibitor control