Fig. 4
From: MiR-135-5p promotes osteoblast differentiation by targeting HIF1AN in MC3T3-E1 cells
HIF1AN is a target gene of miR-135-5p. a – The predicted binding site between the miR-135-5p and HIF1AN, determined using bioinformatics analysis. b – The luciferase activity of HIF1AN-WT and HIF1AN-MUT treated with miR-135-5p mimic or mimic control. ***p < 0.001 vs. 3′-UTR-MUT. c – The expression of HIF1AN was determined using western blot. ***p < 0.001 vs. mimic control. d – The expression of HIF1AN was determined via RT-qPCR. ***p < 0.001 vs. control; #p < 0.05 vs. mimic control; △△p < 0.01 vs. inhibitor control