Fig. 3From: Costunolide reduces glycolysis-associated activation of hepatic stellate cells via inhibition of hexokinase-2Blockade of aerobic glycolysis contributes to the reduction in HSC activation by costunolide. Primary rat HSCs at passages 3 through 5 were treated with costunolide and/or 2-DG at the indicated concentrations for 24 h. a – Real-time PCR for determining the mRNA expression of α-SMA and collagen I. b – Western blot assay for determining the protein expression of α-SMA and collagen I with quantification of the blots. Statistics: **p < 0.01 vs. controlBack to article page