Fig. 3

SOX2 is required for hypoxia-stimulated NEDD9 transcription and expression. (a&b) Cells transfected with siCtrl or siSOX2 were grown under hypoxia for 2 h, and NEDD9 mRNA (a) or protein levels (b) were examined via qPCR or western blotting analysis. In a, SOX2 and NEDD9 were quantified and normalized against β-actin. *p < 0.05, **p < 0.01. In b, NEDD9 was quantified and normalized against β-actin. *p < 0.05, referring to the difference between cells incubated with or without hypoxia. ^#p < 0.05, referring to the difference between cells transfected with siCtrl or with siSOX2 under hypoxia. (c) Cells were co-transfected with the pGL3-Basic (control) or Luc-NEDD9 reporter and siCtrl or siSOX2, respectively. 48 h later, cell extracts were analyzed for luciferase activity. *p < 0.05