Skip to main content
Fig. 2 | Cellular & Molecular Biology Letters

Fig. 2

From: GRP78 regulates milk biosynthesis and the proliferation of bovinemammaryepithelial cells through the mTOR signaling pathway

Fig. 2

RNAi of GRP78 suppresses milk biosynthesis and cell proliferation. a Western blotting analysis of GRP78 and β-casein in BMECs transfected with siRNA targeting GRP78. Cells transfected with scramble siRNA were used as a negative control (siRNA-NC). b and c Relative fold changes in protein levels (proteins/β-actin)of GRP78 (b) and β-casein (c) determined via western blotting and quantified using grayscale scanning. d Triglyceride (TG) contents in the culture medium. e Lactose contents in the culture medium. f Cell numbers measured using a cell counter. g, h and i Cell cycle transition measured (g) and analyzed (h and i) via flow cytometry. Data represent the means ± SE from three independent experiments. *p < 0.05;**p < 0.01

Back to article page

Cellular & Molecular Biology Letters

ISSN: 1689-1392

Contact us