Fig. 3

Overexpression of GRP78 promotes milk biosynthesis and cell proliferation. a Western blot analysis of GRP78 and β-casein in BMECs transfected with pcDNA3.1-GRP78 vector. Cells transfected with the empty vector (pcDNA3.1) were used as negative controls. b and c Relative fold change of protein levels (proteins/β-actin) of GRP78 (b) and β-casein (c) determined via western blotting and quantified using grayscale scanning. d Triglyceride (TG)contents in the culture medium. e Lactose contents in the culture medium. f Cell numbers were measured using a cell counter. g, h and i Cell cycle transition measured (g) and analyzed (h and i) via flow cytometry. Data represent the means ± SEfrom three independent experiments. *p < 0.05;**p < 0.01