Fig. 2

Effects of miR-582–3p overexpression on cell proliferation and cell cycle progression in AML cells. THP-1 cells were transfected with miR-582–3p mimics or miR-NC. a The expression of miR-582–3p was determined via quantitative real-time PCR. b CCK8 assay was performed to evaluate cell proliferation at the indicated times following transfection. c Representative images from the colony formation assay (upper) and quantification of colonies (below) with miR-582–3p mimics or miR-NC-transfected THP-1 cells. d THP-1 cells were stained with PI and subjected to flow cytometry analysis, followed by quantification of cell cycle distribution. Data are presented as means ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 vs. miR-NC